การผลิตเชื้อเห็ดนางรมแบบง่าย

Abstract

The mother culture of mushroom requires adequate equipment which may not be afforded by small laboratories or producers. In this work, simple sterile cultures for mother culture of oyster mushroom (Pleurotus ostreatus) using direct application of hydrogen peroxide (H2O2) disinfectants by preparing sterile medium without autoclaving and inoculating explants without the laminar air-flow cabinet were developed. Contamination trend to decrease when increased percentage of H2O2. The level of culture contamination was within acceptable limits in 0.45% w/v H2O2 treatment. The method is suitable for the preparation of both agar media and Job’ tear media. Growth and development comparison of oyster mushroom with two different mythologies: a traditional methodology and a new methodology were also studied. The results showed that mycelium grew faster in the new methodology; moreover, it took a shorter time for producing fruiting body when compared with a traditional methodology. Both methods gave no significantly different in yield (fresh weight and basic characteristics). Thus, contamination could be reduced without the use of expensive equipment by using this simple method.